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To establish a human colon cancer cell line HBOCS and characterize its biological features. A normal human colon mucosal cell line CT-26 was used as the “normal” control, and a human colon cancer cell line HCT116 was used as the “cancer” control. Immunocytochemistry was performed using antibodies against ABCG2, MDR1, E-cadherin and β-catenin. MicroRNA expression was detected by real-time polymerase chain reaction (PCR). The expression of ABCG2, MDR1, E-cadherin and β-catenin in CT-26 and HBOCS cells were relatively lower and higher than HCT116 cells, respectively. Real-time PCR demonstrated that the microRNA miR-183 was significantly down-regulated in CT-26 and HBOCS cells, and its expression in HBOCS was only about half that in CT-26. The CT-26 cell line was selected as the normal control and the HCT116 as the cancer control. The colorectal cancer cell lines HBOCS may be useful in research into the molecular basis of colon cancer.Maternal Obesity and Birth Weight.
Obesity is a global epidemic that has been associated with increased risk of premature delivery, preterm labor, gestational diabetes, macrosomia, and small for gestational age (SGA) newborns. To date, most studies focused on association between maternal obesity and infant mortality, SGA or macrosomia, as the most common
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