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There is no question that if you have a 3D printer, you can create your own items with it. Free 3D printing is a clever way to learn how to use that.Steroid receptor-independent mechanism for androgen regulation of class III beta-tubulin expression.
Class III beta-tubulin is a developmentally regulated, estrogen-responsive protein that has been implicated in the malignant progression of several human cancers. Its high expression correlates with poorly differentiated human cancers and with the presence of antiestrogen-binding sites, suggesting that androgen receptor (AR) mediates estrogenic induction. However, recent studies indicated that steroids, including androgens, can act as mammary tumor promoters in mice lacking functional AR. Therefore, the hypothesis that AR is responsible for class III beta-tubulin expression was tested in AR-positive human breast cancer cell lines (T-47D and ZR-75-1). Estradiol and the AR agonist R1881 increased class III beta-tubulin expression to a similar extent in these cells, indicating that AR-mediated transcription is not involved in the regulation of class III beta-tubulin expression by estrogens. However, AR antagonists and solvent did not inhibit estradiol-induced class III beta-tubulin expression. In addition, castration did not decrease basal or estradiol-induced class III beta-tubulin expression, suggesting that endogenous androgens are not involved in the regulation of class III beta-tubulin expression in these cells. Because tubulin is known to be an androgen-responsive gene in the prostate, the effect of androgens on class III beta-tubulin expression was also examined in a normal prostatic cell line (BPH1). The result was similar to that observed in breast cancer cells, suggesting that androgens do not regulate class III beta-tubulin expression in prostate cells, perhaps because AR activity is below a critical threshold in these cells.The more I can give to the world, the better I am?
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